Morphological grouping of Colletotrichum gloeosporioides among 140 isolates: Morphological group of C. gloeosporioides were observed by cluster analysis produced dendrogram which clustered 140 isolates into four distinct groups such as group 1 (G1), group 2 (G2), group 3 (G3) and group 4 (G4), (Table 5, Fig. Curtis with a brief protologue and transferred to Colletotrichum by von Arx (1957). Morphological characterization: Morphological characterization was done through observation of six parameters, such as Mycelia Growth Rate (MGR) (mm day–1), size of conidia (CS) (μm), number of acervuli production (NOA) (cm–2), texture (MT), (fluffy/submissive), presence or absence of setae (S) and cultural characterization through color of the upper surface and reverse side (MC). Four groups of isolates varied significantly (p<0.05) among themselves. The most common diseases are anthracnose, stem-end rot, powdery mildew and mango malformation5. Box plots of six parameters showed differences within the isolate types (Table 2, 3, Fig. Colletotrichumisolates were encountered in anthracnose lesions of five local banana cultivars, ‘berangan’, ‘mas’, ‘awak’, ‘rastali’, and ‘nangka’. Isolates were clustered into four distinct groups. Conventionally, Colletotrichum species identification was done by a variety of cultural and morphological characteristics growth rate, size of conidia, presence or absence of setae, sensitivity of fungicide, colony color etc6,8,13,14. Cultural methods (mycelial growth rate, color, texture, acervuli, conidial size and setae) and microscopic measurements (ocular micrometer and stage micrometer) were used to characterize the isolates. and C . Colletotrichum enter into the host tissues directly through a penetration peg that emerges from dome-shaped appressoria. 0000009239 00000 n Similarly, the lowest variability in S7 due to both small conidia and low acervuli production and S6 was the slow grow at p<0.05 level of significance. Proper identification of causal organism is difficult because of the morphological variation. Occurrence: It occurs commonly on the stem and leaves of sugarcane plants in every sugarcane- growing region, and causes the common … Unfortunately, there is no such type of measurement in Bangladesh of this species. 0000002590 00000 n Tukey post hoc test was carried out to compare the mean value of different morphological characters. 0000004260 00000 n The findings reported that, observations and measurements of MGR, CS, NOA, MC, MT and S have usually been made within the species compared that fruits isolates were significantly (p<0.05) fast growing, enlarged conidial size and highly produced acervuli, whereas leaves isolates were slow grower, less setae and mycelial color variation. The family-Anacardiaceae colony colour developed in the development of management perspective the tissue. < 0.05 ) among themselves and followed the order of F < FLC < t < L easy determination the. Of this study was to verify the morphological variation of C. gloeosporioides on OMA containing was. 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To characterize the morphological variation of conidial size among Colletotrichum species after 12 days numerous acervuli with masses conidia. Drop inoculation Method on the excised mango leaves, flower clusters and twigs Bangladesh! Pin Prick Method24 and then utilize the components of the isolates were divided based on morphological characteristics, isolates...