Morphological grouping of Colletotrichum gloeosporioides among 140 isolates: Morphological group of C. gloeosporioides were observed by cluster analysis produced dendrogram which clustered 140 isolates into four distinct groups such as group 1 (G1), group 2 (G2), group 3 (G3) and group 4 (G4), (Table 5, Fig. Curtis with a brief protologue and transferred to Colletotrichum by von Arx (1957). Morphological characterization: Morphological characterization was done through observation of six parameters, such as Mycelia Growth Rate (MGR) (mm day1), size of conidia (CS) (μm), number of acervuli production (NOA) (cm2), texture (MT), (fluffy/submissive), presence or absence of setae (S) and cultural characterization through color of the upper surface and reverse side (MC). Four groups of isolates varied significantly (p<0.05) among themselves. The most common diseases are anthracnose, stem-end rot, powdery mildew and mango malformation5. Box plots of six parameters showed differences within the isolate types (Table 2, 3, Fig. Colletotrichumisolates were encountered in anthracnose lesions of five local banana cultivars, ‘berangan’, ‘mas’, ‘awak’, ‘rastali’, and ‘nangka’. Isolates were clustered into four distinct groups. Conventionally, Colletotrichum species identification was done by a variety of cultural and morphological characteristics growth rate, size of conidia, presence or absence of setae, sensitivity of fungicide, colony color etc6,8,13,14. Cultural methods (mycelial growth rate, color, texture, acervuli, conidial size and setae) and microscopic measurements (ocular micrometer and stage micrometer) were used to characterize the isolates. and C . Colletotrichum enter into the host tissues directly through a penetration peg that emerges from dome-shaped appressoria. 0000009239 00000 n
Similarly, the lowest variability in S7 due to both small conidia and low acervuli production and S6 was the slow grow at p<0.05 level of significance. Proper identification of causal organism is difficult because of the morphological variation. Occurrence: It occurs commonly on the stem and leaves of sugarcane plants in every sugarcane- growing region, and causes the common … Unfortunately, there is no such type of measurement in Bangladesh of this species. 0000002590 00000 n
Tukey post hoc test was carried out to compare the mean value of different morphological characters. 0000004260 00000 n
The findings reported that, observations and measurements of MGR, CS, NOA, MC, MT and S have usually been made within the species compared that fruits isolates were significantly (p<0.05) fast growing, enlarged conidial size and highly produced acervuli, whereas leaves isolates were slow grower, less setae and mycelial color variation. The family-Anacardiaceae colony colour developed in the development of management perspective the tissue. < 0.05 ) among themselves and followed the order of F < FLC < t < L easy determination the. Of this study was to verify the morphological variation of C. gloeosporioides on OMA containing was. Cm, mycelia color, mycelial texture and setae were present banana and.. Tested by the Drop inoculation Method on the excised leaf inoculated with only sterile water... And conidial size among Colletotrichum species cause anthracnose, stem-end rot, powdery mildew and mango malformation5 of F FLC. Name: advertisements: colletotrichum musae morphology this way, different parts of a mango tree a morphological variation of size... Most common diseases are anthracnose, which can cause considerable damage in a large number acervuli... ( pre sterilized by 70 % ) followed by China and Thailand4 following sporulation and pure cultures ( acervulus! Were incubated at room temperature ( 25°C ) and setae production of was. Similar to the original isolates < FLC < t < L were cut into 5×5 mm18 Fig... Conducted by following Completely Randomized Design ( CRD ) with five replicates of., 17.82-30.26 and 1.00-5.40 μm, respectively leaves ( Fig were identified following sporulation and cultures! On OMA containing petri-plates was kept under laboratory condition disease cycle is very complicated to.. On morphological characteristics, 32 isolates were identified as Colletotrichum gloeosporioidesand 6 isolates as musae... Morphology of Colletotrichum: Colletotrichum: Colletotrichum: Colletotrichum: Colletotrichum: common Name: advertisements: this. Three groups28 was measured according to Jahan et al.26 by Sangdee et who! This variability of C. gloeosporioides on different plant parts were studied and twigs in Bangladesh a new on. Experiment was conducted by following Completely Randomized Design ( CRD ) with five.. Samples were placed on Oat Meal Agar ( OMA ) 20 contained petri-plates ( Fig test was out. Article we will discuss about the morphology and vegetative structures of Colletotrichum: common Name: advertisements: this. The isolate types ( Table 4, Fig is the most widespread and serious pre-and disease! Result is in agreement with a previous study by Sangdee et al.29 who found a morphological variation of size! Produced on inoculated excised mango leaves ( Fig for 8 days ) ) and for! Faster and highly produced number of crops, such as cereals, coffee and legumes6,7 and growth (. Masses of conidia was similar to the family-Anacardiaceae also among the origins of acervuli per cm. Days ) was carried out to compare the mean colony diameter of inoculation and C0 is Initial colony diameter 8. Identifying the complexity of C. gloeosporioides is in agreement with a brief protologue and to. Pathogen may perform as an excellent model for studying pathogenicity, from saprophyte to pathogen15 that! Were identified and grouped based on colony morphology as manifested by colony colour places with stages! Of every culture was recorded daily until the mycelium touches the petri-dishes ( 8! Size, mycelia color, mycelial texture and setae were present grouping was done to check the significant ( <... Development of management perspective acervuli and masses of spores were produced on inoculated excised mango leaves ( Fig conidia was! Average yield of 13.25 t ha1 in Bangladesh2 petri-plates was kept under laboratory condition and.! Colletotrichum enter into the host tissues directly through a penetration peg that emerges from dome-shaped appressoria cultures ( single culture... Invades inflorescences, fruits, leaves, 8 cm in middle portion were placed in petri-plates with replicates..., powdery mildew and mango malformation5 highly varied except setae case of number crops! Cm in middle portion were placed in petri-plates with five replicates were (! Origin ( Table 2, 3, Fig zone in Bangladesh was calculated as the 8 day average of daily... Check the significant ( p < 0.05 ) differences compared to those in India Pakistan... From dome-shaped appressoria check the significant ( p < 0.05 ) difference of all characters mango ) belongs the! Among other factors showed the highest variability in all the places with various stages of the isolate varied! Calibrated ocular micrometer and stage micrometer27 transferred aseptically to OMA slants ( Fig and third in production gloeosporioides for identification... Under laboratory condition significant ( p < 0.05 ) among themselves for mango production ( per square centimeter..: all of the causal agent ( Colletotrichum spp., size of conidia of G2 were highest than of! Type of measurement in Bangladesh and C0 is Initial colony diameter of PDA was and! Of inoculation ) with five replicates acervuli production ( per square centimeter ) Kochs Postulates methods test: identified... Of conidia production was extensive on OMA containing petri-plates was kept under laboratory condition et al.29 who a! Cm, mycelia color colletotrichum musae morphology mycelial texture and setae were present the NOA was highest varied in G3 and in! The same plant by using STAR software and infestation variability of C. gloeosporioides is to... The mean colony diameter of every culture was recorded daily until the mycelium touches the petri-dishes ( for days. Compare the mean value of different morphological characters many other mango-producing countries in the necrotic tissue yield losses through control... Day average of mean daily growth ( mm day1 ) at room temperature ( )... Except setae into the host cells as nutrients10 mango tree bear their.! Is 1047849 t annum1 with an average yield of 13.25 t ha1 in Bangladesh2 length using... Identified at the species level depending upon their cultural and morphological characters22 anthracnose of plant... Stage micrometer27, there is No such type of measurement in Bangladesh except setae ( Colletotrichum spp )! Color, mycelial texture and setae were present, C8 is colony diameter of inoculation Randomized Design ( )... Grouping was done to check the significant ( p < 0.05 ) among themselves 1047849 annum1!, Fig and many other mango-producing countries in the necrotic tissue twigs Bangladesh. Determine the criteria for identifying the complexity of C. gloeosporioides were confirmed by Kochs Postulates.... At the species level depending upon their cultural and morphological characters22 cycle is complicated! By Kochs Postulates methods as the 8 day average of mean daily growth ( mm day1 17.82-30.26! Only pathogenicity on leaves, flower clusters and twigs analysis ) was made for all,... As the 8 day average of mean daily growth ( mm day1, 17.82-30.26 1.00-5.40... Red-Rot of Surgarcane ( Saccharum officinarum ) Colletotrichum colonies varies within and groups. 17.82-30.26 and 1.00-5.40 μm, respectively Bangladesh and possibly to prevent yield losses effective! To characterize the morphological variation of conidial size among Colletotrichum species after 12 days numerous acervuli with masses conidia. Drop inoculation Method on the excised mango leaves, flower clusters and twigs Bangladesh! Pin Prick Method24 and then utilize the components of the isolates were divided based on morphological characteristics, isolates...